Abstract: The RFID system is susceptible to a variety of factors such as metal, liquid and so on in the practical application environment. Therefore, the influence of metal on the dynamic performance of typical RFID system was analyzed theoretically, including reading efficiency and reading distance. A kind of RFID dynamic performance testing system based on photoelectric sensor was explained, which consisted of a conveyor belt, data acquisition and processing system, high accuracy ranging system and so on. The experiment of dynamic data acquisition and verification based on the system was also illustrated. The experimental results showed that metal had a significant influence on the dynamic performance of RFID system but the dynamic reading and anti-interference ability could be improved by some particular methods. The work supplied an effective way for dynamic data acquisition and measurement of RFID system.