Abstract: Now digital photographing technology and image analyse technology are setted to the metallugical microscope for microstucture test and analysis. According to the ISO/IEC 17025∶2005 and combining the standard methods of microstructure test. This paper gives several methods to validate these new methods by analyzing images of stage micrometer,metallurgical sample,and images that consisting of definite area in geometric graph,Several kinds of validation methods including of traceability of a quantity value,printing microstucture graph,comparing microstucture photo with standard photos,length meansurement,and area meansurement are discussed in this paper. These methods also can be used to check and accept quantiative image analysis system or new digital photomicrography system installed in the metallugical microscope.