Abstract: Based on the study and calculation of Kikuchi line of transmission electron microscopy, The potential application of Kikuchi line was developed, and a new method of small angle grain boundary measurement using Kikuchi line of transmission electron microscopy was established. The results show that the angle between different grains could be calculated effectively by using the Kikuchi center of different grains. In addition, the deviation of the angle value was very small (the relative deviation was only 2.88%) under different conditions of Kikuchi line, which had good consistency.